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Regulations

Many international agencies are trying to achieve universal standardisation of regulatory limits for mycotoxins. This is an incredibly difficult task as many factors have to be considered when deciding on regulatory standards. In addition to scientific factors, such as risk assessment and analytical accuracy, economical and political factors such as the commercial interests of each country and the constant necessity of a sufficient food supply also play a role in the decision making process.

The whole process is further complicated by the fact that action levels pertain to single mycotoxin contamination, but in reality, several mycotoxins often occur in combination which may require different and often lower action levels.

Measuring the toxicological effects of a variety of different mycotoxin combinations, as they occur in nature, is an enormous and probably impossible task, especially considering that they may be mycotoxins present that have not been identified. In addition, nutritional, management, environmental and species all play a contributory role in determining the effect of a combination of mycotoxins on animal health.

Despite these challenges most countries within the European Union have come to a common agreement on a standardised policy for the regulation of the main mycotoxin levels in different feedstuffs and feedstuff ingredients.

The ideal goal is to eliminate mycotoxins from the food chain. However, on a practical level, this is not possible. The tolerance levels in this section are based mainly on studies of individual toxins. Further research into the interactions of mycotoxins with each other and with other environmental and nutritional factors will enable validation and modification of these guidelines.




Europe

EU
Commission Recommendation of 17th August 2006 on the presence of deoxynivalenol, zearalenone, ochratoxin A, T-2 and HT-2 and fumonisins in products intended for animal feeding (2006/576/EC)

The European Commission has just announced its recommendations on the presence of deoxynivalenol (DON), zearalenone (ZEA), ochratoxin A (OTA), T-2 and HT-2 and fumonisins in products intended for animal feeding.

The key points from this Recommendation are:

  • Increase monitoring for the presence of DON, ZEA, OTA, fumonisin B1 & B2, T-2 and HT-2 toxin in cereals and cereal products intended for animal feeding and compound feeding stuffs.
  • Ensure that samples are simultaneously analysed for the presence of DON, ZEA, OTA, fumonisin B1 & B2, T-2 and HT-2 toxin to allow the extent of co-occurrence to be assessed.
  • Pay particular attention to the presence of those mycotoxins in by- or co-products from the production of food intended for animal feeding.
  • Ensure that analytical results are provided on a regular basis to the Commission for compilation into a single database.
  • Ensure that feed business operators use in their HACCP system the guidance values.
  • For feed for more sensitive animals, ensure that lower guidance values for cereals and cereal products are applied by feed manufacturers taking into account the sensitivity of the animal species and enabling compliance with the guidance values determined for compound feedingstuffs for these animal species.

Mycotoxins Products intended for animal feed Guidance value in mg/kg (ppm) relative to a feedingstuff with a moisture content of 12%
Deoxynivalenol Feed materials (*)
Cereals and cereal products (**) with the exception of maize by-products 8
Maize by-products 12
Complementary and complete feedingstuffs with the exception of: 5
Complementary and complete feedingstuffs for pigs 0.9
Complementary and complete feedingstuffs for calves (<4 months), lambs and kids 2
Zearalenone Feed materials (*)
Cereals and cereal products (**) with the exception of maize by-products 2
Maize by-products 3
Complementary and complete feedingstuffs with the exception of:
Complementary and complete feedingstuffs for piglets and gilts (young sows) 0.1
Complementary and complete feedingstuffs for sows and fattening pigs 0.25
Complementary and complete feedingstuffs for calves, dairy cattle, sheep (including lamb) and goats (including kids) 0.5
Ochratoxin A Feed materials (*)
Cereals and cereal products (**) 0.25
Complementary and complete feedingstuffs: 5
Complementary and complete feedingstuffs for pigs 0.05
Complementary and complete feedingstuffs for poultry 0.1
Fumonisin B1 + B2 Feed materials (*)
Maize and maize products (***) 60
Complementary and complete feedingstuffs for:
Pigs, horses, rabbits and pet animals 5
Fish 10
Poultry, calves (<4months), lambs and kids 20
Adult ruminants (>4months) and mink 50

(*) Particular attention has to be paid to cereals and cereals products fed directly to the animals that their use in a daily ration should not lead to the animal being exposed to a higher level of these mycotoxins that the corresponding levels of exposure where only the complete feedingstuffs are used in a dairy ration.
(**) The term 'cereals and cereal products' includes not only the feed materials listed under heading 1 'Cereal grains, their products and by-products of the non-exclusive list of main feed materials referred to in part B of the Annex to Council Directive 96/25/EC of 29 April 1996 on the circulation and use of feed materials (OJ L 125, 23.5.1996, p.35) but also other feed materials derives from cereals in particular cereal forages and roughages.
(***) The term 'Maize and maize products' includes not only the feed materials derived from maize listed under heading 1 'Cereal grains, their products and by-products' of the non-exclusive list of main feed materials referred to in the Annex, part B of Directive 96/25/EC but also other feed materials derived from maize in particular maize forages and roughages.


DIRECTIVE 2002/32/EC OF THE EUROPEAN PARLIAMENT AND OF THE COUNCIL of 7 May 2002 on undesirable substances in animal feed

Products intended for animal feed may contain undesirable substances which can endanger animal health or, because of their presence in livestock products, human health or the environment.

These regulations apply to products intended for animal feed as soon as they enter the EU.

Aflatoxin B1

Products intended for animal feed Max. content in mg/kg (ppm) relative to a feedingstuff with a moisture content of 12%
Complete feeding stuffs for cattle, sheep and goats with the exception of: 0.05
Dairy Cattle 0.005
Calves and lambs 0.01


Rye Ergot (Claviceps pupurea)

Products intended for animal feed Max. content in mg/kg (ppm) relative to a feedingstuff with a moisture content of 12%
All feeding stuffs containing unground cereals 1000


Aflatoxins

Max level aflatoxin in μg/kg (ppb)
Product B1 B1, B2, G1, G2
Cereals and processed products thereof intended for direct human consumption or use as an ingredient in foodstuffs 2.0 4.0
Cereals with the exception of maize, to be subjected to sorting or other physical treatment, before human consumption or use as an ingredient in foodstuffs 2.0 4.0
Maize to be subjected to sorting, or other physical treatment, before human consumption or use as an ingredient in foodstuffs 5.0 10.0





Non EU Countries

Country Commodity (Sum of) Mycotoxin(s) Limit (ug/kg)
Serbia & Montenegro Feed for chicken, pigs (<50kg), calves, young turkeys, ducklings, cows Aflatoxin B1, B2, G1, G2 10
Feed for ox, sheep, goat Aflatoxin B1, B2, G1, G2 50
Feed for swine and poultry Aflatoxin B1, B2, G1, G2 20
Feed for pigs (<50kg) Ochratoxin A 100
Feed for swine Ochratoxin A 200
Feed for poultry Ochratoxin A 1000
Feed for layers Ochratoxin A 250
Feed for chickens and pigs (<50kg) and calves Tricothecenes 300
Feed for swine ox and poultry Tricothecenes 600
Feed for pigs (<50Kg) Zearalenone 500
Feed for other swine Zearalenone 1000
Feed for cows, sheep and goats Zearalenone 3000
Feed for ox Zearalanone 5000
Feed for layers Zearalenone 100000
Switzerland Babassu seed, cotton seed, peanut, coconut, maize kernel, palm kernel and their products as raw materials Aflatoxin B1 200
Babassu seed, cotton seed, peanut, coconut, maize kernel, palm kernel and their products as single feed materials Aflatoxin B1 20
Other single feeds/raw materials Aflatoxin B1 50
Complete and complementary feeds for bovine animals, sheep and goats, except milk cows, calves and lambs Aflatoxin B1 50
Complementary feeds for pigs and poultry except young animals Ochratoxin B1 30
Complete feeds for pigs and poultry except young animals Ochratoxin B1 20
Complementary feed for lactating bovine animals, lactating sheep and lactating goats Ochratoxin B1 5
Other complete and complementary feeds Aflatoxin B1 10
Turkey Feed ingredients Aflatoxin B1 50
Mixed feed for ruminants except young animals Aflatoxin B1 50
Mixed feed for poultry except young animals Aflatoxin B1 20
Other mixed feed Aflatoxin B1 10
Ukraine Combined feed for non-productive animals Aflatoxin B1 10
Combined feed for poultry Aflatoxin B1 25
Combined feed for cows in the period of lactation, for piglets no older than 2 months Aflatoxin B1 50
Combined feed for calves and sheep older than 4 months, animals for meat and breeding bulls Aflatoxin B1 100
Combined feed for sows (pregnant, feeding), breeding boars, piglets younger than 2 months Zearalenone 40
Soya press for feed Zearalenone 1000
Combined feed for pigs fed for pork lighter than 50kg weight Zearalenone 2000
Combined feed for pigs fed for pork over 50kg weight Zearalenone 3000
Combined feed for all animals DON 1000
Combined feed for layers and broilers T-2 toxin 200
Combined feed for calves and older cattle fed for beef T-2 toxin 250





Latin America

Country Commodity (Sum of) Mycotoxin(s) Limit (ug/kg)
El Salvador All feedstuffs Aflatoxin B1 10
Supplementary feeds for porcine/poultry/dairy cattle; single composite feedstuffs; bovine/caprine/ovine feedstuffs Aflatoxin B1 20
Suriname Feedstuffs Aflatoxin B1, B2, G1, G2 30
Uruguay DON Ochratoxin A 5000
DON Ochratoxin A 2000
DON Ochratoxin A 1000
DON Ochratoxin A 2000
Animal feed Ergot alkaloids 450
Animal feed for pigs and female rabbits Ergot alkaloids Not detectable
Barbados All feedstuffs Aflatoxin B1 B2G1G2 50
Brazil Animal feed and ingredients: hay cotton, peanut, rice, oats, residues of bird bowels, babassu, cocoa, sugar cane (residue-pulp), linhaca, dende, manioc, sunflower, crisálidas, malt, wheat, soya, yeast (sugar cane subproduct) Aflatoxin B1 B2G1G2 50
Chile Complete feedingstuffs for poultry, goats, goats and cattle Aflatoxin B1 B2 G1 G2 30
Complete feedingstuffs for other animals Aflatoxin B1 B2 G1 G2 10
All ingredients for use in animal feed except peanuts and derivatives, cottonseed and derivatives, maize and derivatives Aflatoxin B1 B2 G1 G2 50
Peanuts and derivatives, cottonseed and derivatives, maize and derivatives Aflatoxin B1 B2 G1 G2 200
Colombia Maize and maize products Aflatoxin B1 B2 G1 G2 20
Sorghum Aflatoxin B1 B2 G1 G2 40
Rabbit/Trout feeds Aflatoxin B1 B2 G1 G2 10
Poultry, dog, cat, fish feeds Aflatoxin B1 B2 G1 G2 20
Bovine, pig feeds Aflatoxin B1 B2 G1 G2 50
Sorghum Zearalenone 1000
Costarica Maize Aflatoxin B1 B2 G1 G2 50
Cuba All feeds and feed ingredients Aflatoxin B1 B2 G1 G2 5
All feeds DON 300
Mexico Cereals for bovine and porcine fattening feedstuffs Aflatoxin B1 B2 G1 G2 200
Feedstuffs for dairy cattle/poultry Aflatoxin B1 B2 G1 G2 0
Venezuela Feeds Aflatoxin B1 B2 G1 G2 20





Asia Pacific

Country Commodity (Sum of) Mycotoxin(s) Limit (ug/kg)
Taiwan Maize (raw materials) Aflatoxin B1, B2, G1, G2 50
All Feedstuffs Aflatoxin B1 20
Bangladesh Maize and mixed feed for poultry Aflatoxin B1 B2G1G2
Maize and mixed feed for poultry Ochratoxin A
China Complete feed Aflatoxin B1
Ochratoxin
Fumonisins
T-2 toxin
Zearalenone
Vomitoxin
20
20
500
80
100
500
Corn, peanut meal, peanut cake, rapeseed meal, cotton seed meal Aflatoxin B1 <=50
Soybean meal Aflatoxin B1 <=30
Complete feed and concentrate for piglets Aflatoxin B1 <=10
Complete feed and concentrate for sows and grower/finisher pigs Aflatoxin B1 <=20
Young broilers pre-starter complete feed and concentrate Aflatoxin B1 <=10
Young broilers starter, grower and layer complete feed and concentrate Aflatoxin B1 <=20
Young meat ducks pre-starter complete feed and concentrate Aflatoxin B1 <=10
Young meat ducks starter, grower and layer ducks complete feed and concentrate Aflatoxin B1 <=15
Quails complete feed and concentrate Aflatoxin B1 <=20
Dairy cows concentrate Aflatoxin B1 <=10
Beef cattle concentrates Aflatoxin B1 <=50
India Peanut meal (export) Aflatoxin B1 120
Japan Compound feeds for cattle (except calves, dairy cows), pigs (except piglets), chicken (except young chicken, broilers) quails Aflatoxin B1 20
Compound feeds for calves, dairy cows, piglets, young chicken and broilers Aflatoxin B1 10
Compound feeds Zearalenone
DON
1000
1000
Compound feeds for cows with an age over 3 months DON 4000
Complete feed for beef cow (excluding calves), swine (excluding piglet), poultry (excluding chicks and quail) Aflatoxin B1 20
Complete feed for calves, dairy cows, piglets, chicks and broiler starters Aflatoxin B1 200
Feed for all livestock Zearalenone 20
Feed for all livestock (excluding calves <3months) DON 200
Feed for calves <3months DON 4000
Korea Compound feeds for calves, chicken, piglets, broilers (early stage) and dairy cattle Aflatoxin B1 B2 G1 G2
Ochratoxin A
10
200
Other compound feeds (except premixes) Aflatoxin B1
Ochratoxin A
2
2000
Feed ingredients: vegetable proteins, grains, by-products of grains and food Aflatoxin B1 50
Nepal Feedstuffs Aflatoxin B1 50
The Philippines Mixed Feed Aflatoxin B1 20
Copra and copra products Aflatoxin B1 20
Commercial feeds Aflatoxin 25
Indonesia Copra rations for cows, swine ducks and sheep Aflatoxin B1 B2 G1 G2 1000
Peanut sesame and colza flowers Aflatoxin B1 B2 G1 G2 200
Cassava in chicken rations Aflatoxin B1 B2 G1 G2 200





N. America

Country Commodity (Sum of) Mycotoxin(s) Limit (ug/kg)
USA Corn and peanut products intended for finishing (i.e. feedlot) beef cattle Aflatoxin B1, B2, G1, G2 300
Cottonseed meal intended for beef cattle, swine, or poultry Aflatoxin B1, B2, G1, G2 300
Corn or peanut products intended for finishing swine of 100 pounds or greater Aflatoxin B1, B2, G1, G2 200
Corn and peanut products intended for breeding beef cattle, breeding swine, or mature poultry Aflatoxin B1, B2, G1, G2 100
Corn, peanut products, and other animal feeds and feed ingredients excluding cottonseed meal, intended for immature animals Aflatoxin B1, B2, G1, G2 20
Corn, corn products, cottonseed meal, and other animal feeds and feed ingredients intended for dairy animals, for animal species or uses not specified above, or when the intended use is not known. Aflatoxin B1, B2, G1, G2 20
For grain and grain
co-products destined for swine. FDA recommends that ingredients containing this level not to exceed 20 percent of the diet.
DON 5000
For grain and grain co-products destined for ruminanting beef cattle and feedlot cattle older than four months. FDA further reccommends that this 10 p.p.m. level not be exceeded in the total ration (includes all grains; grains co-products, including distillers and brewers grains; gluten feed and gluten meals; hay; silage; and roughage). DON 10000
For grain and grain co-products destined for ruminanting dairy cattle older than four months. FDA further recommends that DON not exceed 5 p.p.m. of the total ration (includes all grains; grains co-products, including distillers and brewers grains; gluten feed and gluten meals; hay; silage; and roughage). DON 10000
For distillers grains, brewers grains, gluten meals destined for ruminating beef and feedlot cattle, as well as ruminating dairy cattle older than four months. FDA further recommends that DON not exceed 10 p.p.m. in the total ration for beef and feedlot cattle, and 5 p.p.m. in the toal ration for dairy cattle. DON 30000
For grain and grain co-products destined for chickens. FDA further reccomends ingredients at this level not exceed 50 percent diet. DON 10000
For grain and grain byproducts destined for all other animal species. FDA further recommends that ingredients containg this level not exceed 40 percent of the diet. DON 5000
Corn and corn by-products intended for equids and rabbits Fumonisins B1, B2, B3 5000
Corn and corn by-products intended for swine and catfish Fumonisins B1, B2, B3 20000
Corn and corn by-products intended for breeding ruminants, breeding poultry and breeding mink (includes lactating dairy cattle and layers) Fumonisins B1, B2, B3 30000
Ruminants >3months old being raised for slaughter and mink being raised for pelt production Fumonisins B1, B2, B3 60000
Poultry for slaughter Fumonisins B1, B2, B3 100000
All other species or classes of livestock and pet animals Fumonisins B1, B2, B3 10000
Canada All feeds Aflatoxin B1 B2G1G2 20
Feed for swine and poultry Ochratoxin A
T-2 toxin
2000
1000
Feed for cattle and poultry DON
HT-2 Toxin
5000
100
Feed for swine, calves, dairy cattle DON 1000
Feed for gilts and sows Zearalenone 3000
Feed for swine Diacetoxyscirpenol
Ergot alkaloids
2000
6000
Feed for poultry Diacetoxyscirpenol 1000
Feed for cattle, sheep, horsesp Ergot alkaloids 3000
Feed for chick Ergot alkaloids 9000





Africa & Middle East

Country Commodity (Sum of) Mycotoxin(s) Limit (ug/kg)
Senegal Peanut products (straight feedstuffs) Aflatoxin B1 50
Peanut products (feedstuffs ingredients) Aflatoxin B1 300
Syrian Arab Republic Domestic Feed AAflatoxin B1 B2 G1 G2 20
Livestock cattle feed Aflatoxin B1 B2 G1 G2 10
Tanzania Feeds Aflatoxin B1
Aflatoxin B1, B2, G1, G2
5
10
Cote d'Ivoire Straight feedstuffs Aflatoxin B1 B2 G1 G2 100
Complete feedstuffs Aflatoxin B1 B2 G1 G2 10
Complete feedstuffs for pigs, poultry except your animals/ducks Aflatoxin B1 B2 G1 G2 38
Complete feedstuffs for cattle, sheep, goats Aflatoxin B1 B2 G1 G2 75
Complete feedstuffs for dairy cattle Aflatoxin B1 B2 G1 G2 50
Egypt Animal and chicken feed Aflatoxin B1
Aflatoxin B1 B2 G1 G2
10
20
Iran Cotton seed meal Aflatoxin B1
Aflatoxin B1 B2 G1 G2
15
20
Fish meal, meat meal, bone meal, blood meal, single cell protein, rice and wheat bran intended for sheep, goats and beef cattle Aflatoxin B1 10
Fish meal, meat meal, bone meal, blood meal, single cell protein, rice and wheat bran intended for poultry, calf, lamb, kid, dairy sheep, goats and dairy Aflatoxin B1
Aflatoxin B1 B2 G1 G2
5
20
Soya bean meal, sunflower meal, sesame seed meal, olive meal and other meals from oil producing seeds intended for sheep, goats and beef cattle Aflatoxin B1
Aflatoxin B1 B2 G1 G2
10
20
Soya bean meal, sunflower meal, sesame seed meal, olive meal and other meals from oil producing seeds intended for poultry, calf, lamb, kid, dairy sheep, goats and dairy cattle Aflatoxin B1
Aflatoxin B1 B2 G1 G2
5
20
Maize intended for sheep, goats and beef cattle, poultry, calf, lamb, kid, dairy sheep, dairy goats and dairy cattle Aflatoxin B1
Aflatoxin B1 B2 G1 G2
5
20
Premixes including vitamins and mineral premixes intended for sheep, goats and beef cattle Aflatoxin B1 B2 G1 G2 10
Premixes including vitamins and mineral premixes intended for calf, lamb, kid, dairy sheep, goats and cattle Aflatoxin B1 B2 G1 G2 5
Premixes including vitamins and mineral premixes intended for poultry Aflatoxin B1 B2 G1 G2 10
Complete feed intended for sheep, goats and beef cattle Aflatoxin B1
DON
T-2 Toxin
50
5000
100
Complete feed intended for calf, lamb, kid, dairy sheep, goats and cattle Aflatoxin B1
DON
T-2 Toxin
5
1000
25
Complete feed intended for layers and breeders (broilers and layers) Aflatoxin B1
Aflatoxin B1 B2 G1 G2
10
20
Complete feed intended for broilers and pullet Aflatoxin B1 10
Complete feed intended for parent and grandparent stocks Aflatoxin B1
Aflatoxin B1 B2 G1 G2
5
20
Israel All grains Aflatoxin
Ochratoxin A
DON
Zearalenone
T-2 toxin
Diacetoxyscripenol
20
300
1000
Not given
100
200
Jordan All feedstuffs Aflatoxin B1
Aflatoxin B1 B2 G1 G2
15
30
Morocco Simple feedstuffs (except peanuts, Copra, cottonseed, babassu, maize and their products) Aflatoxin B1
Aflatoxin B1 B2 G1 G2
50
Peanuts, copra, cottonseed, babassu, maize and their products Aflatoxin B1 20
Complete feedstuffs for cattle, sheep and goats (except for dairy animals, calves and lambs) Aflatoxin B1 50
Complete feedstuffs for dairy animals Aflatoxin B1 5
Complete feedstuffs for calves and lambs Aflatoxin B1 10
Complete feedstuffs for pigs and poultry (except young animals) Aflatoxin B1 20
Other complete feedstuffs Aflatoxin B1 10
Complementary feedstuffs for cattle, sheep and goats (except for dairy animals, calves and lambs) Aflatoxin B1 50
Complementary feedstuffs for pigs and poultry (except young animals) Aflatoxin B1 30
Other complementary feedstuffs, especially dairy animals Aflatoxin B1 10
Mozambique Peanut, maize, peanut butter Aflatoxin B1 B2 G1 G2 10
Cereals and feedstuffs Aflatoxin B1 B2 G1 G2 10
Feedstuffs Aflatoxin B1 Unknown
Corn Ochratoxin A
Zearalenone
Unknown
Unknown
Nigeria Rations Aflatoxin B1 50
Oman Complete feedstuffs for poultry Aflatoxin B1 20
Zimbabwe Rations for poultry Aflatoxin B1 G1 10

 



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